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在研究核酸分子水化作用的工作中,前人及作者的前文都是将样品保持在恒温器中,在静态情况下实现湿度平衡。为了使样品能更充分地吸附水分子,我们设计了一个通气系统,使不同湿度的水蒸气连续通过密封样品池,让样品在动态情况下达到湿度平衡。除了水化过程外,对脱水过程的观察、分析也是研究核酸与水的相互作用的不可缺少的一环,但这方面的实验工作却很少。我们通过对Poly A薄膜动态水化和脱水过程中红外光谱的测定,证实了:(1)与静
In the work of studying the hydration of nucleic acid molecules, the predecessors and authors previously held samples in a thermostat to achieve a moisture balance under static conditions. In order to allow the sample to adsorb water molecules more fully, we designed a ventilation system that continuously passes water vapor of different humidities through the sealed sample cell to allow the sample to reach a moisture equilibrium under dynamic conditions. In addition to the hydration process, the observation and analysis of the dehydration process is also an indispensable part of the study of the interaction between nucleic acid and water. However, experimental work in this area is rare. Through the determination of the infrared spectra during the dynamic hydration and dehydration of Poly A films, we confirmed: (1)