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目的:比较超速离心法、电泳法、匀相法、沉淀法在干扰因素存在时检测冠心病(CHD)患者血清胆固醇水平的特异性。方法:采用超速离心法、电泳法、匀相法及沉淀法对150例CHD患者和102例健康体检者分别进行血清胆固醇水平的测定,比较各方法之间的相关性,同时分析导致结果差异的因素。结果:四种方法检测健康人群HDL-C、LDL-C水平,结果间无统计学差异(P>0.05);CHD组,当TG≤2.26mmol/L时,采用沉淀法和匀相法检测CHD患者血清LDL-C,结果差异较小(P>0.05),电泳法检测结果偏高,与沉淀法、电泳法间有统计学差异(P<0.05)。超速离心法与电泳法间的相关性良好,但匀相法及沉淀法易受高胆红素、高血红蛋白等因素的影响。结论:超速离心法虽耗时、价格贵,但仍为检测HDL-C、LDL-C的经典方法,电泳法受高胆红素、血红蛋白、高三酰甘油等干扰因素的影响相对较小,适用于冠心病HE合并高血脂血清HDL-C、LDL-C水平检测。
OBJECTIVE: To compare the specificity of serum cholesterol levels in patients with coronary heart disease (CHD) in the presence of interfering factors by ultracentrifugation, electrophoresis, homogenization and precipitation. Methods: The levels of serum cholesterol in 150 CHD patients and 102 healthy subjects were determined by ultracentrifugation, electrophoresis, homogenization and sedimentation respectively. The correlations among different methods were compared, and the differences between the two methods were analyzed factor. Results: There were no significant differences in HDL-C and LDL-C between healthy controls and CHD patients (P> 0.05). CHD group, when TG≤2.26mmol / L, Serum LDL-C, the difference was small (P> 0.05), electrophoresis test results were high, and precipitation and electrophoresis were statistically different (P <0.05). The correlation between ultracentrifugation and electrophoresis was good, but the homogeneous method and precipitation method were susceptible to such factors as high bilirubin and high hemoglobin. Conclusion: Ultracentrifugation is a time-consuming and expensive method, but it is still the classic method for the detection of HDL-C and LDL-C. The electrophoresis method is relatively less affected by the interference factors such as hyperbilirubin, hemoglobin and triglyceride HE in coronary heart disease with hyperlipidemia serum HDL-C, LDL-C levels were detected.