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目的研究人参皂苷Rg1对小鼠腹腔巨噬细胞的影响,并探讨其作用机制。方法无菌分离小鼠腹腔巨噬细胞,制备单细胞悬液,加入不同终浓度的人参皂苷Rg1 4 h后,细菌脂多糖LPS(10μg/ml)作用24 h,直径1μm的微球(1×1010/L)结合流式细胞术分析Rg1对小鼠腹腔巨噬细胞吞噬作用的影响;Griess试剂盒检测NO的释放;H2DCFDA染色检测ROS的含量;Fluo-4/AM染色检测Rg1对Ca2+超载的影响;Sytox R Green染色,荧光酶标仪检测Rg1对CHX、CTX诱导的细胞凋亡的影响。结果 10、20μmol/L的Rg1时能明显抑制LPS诱导的巨噬细胞NO和ROS的产生以及吞噬微球的能力;10μmol/L的Rg1能显著抑制Ion诱导的的巨噬细胞Ca2+的超载以及CHX、CTX诱导的细胞的凋亡。结论 Rg1具有显著的抗炎作用,并可抵抗多种因素诱导的细胞凋亡,为进一步开发为免疫治疗药物提供了依据。
Objective To study the effect of ginsenoside Rg1 on murine peritoneal macrophages and to explore its mechanism. Methods The peritoneal macrophages of mice were aseptically isolated and prepared single cell suspension. After adding different concentrations of ginsenoside Rg1 for 4 h, the bacterial lipopolysaccharide LPS (10 μg / ml) for 24 h and the diameter of 1 μm microspheres (1 × 1010 / L) was used to analyze the effect of Rg1 on phagocytosis of mouse peritoneal macrophages by flow cytometry; release of NO was detected by Griess kit; ROS was detected by H2DCFDA staining; Fluo-4 / AM staining was used to detect the effect of Rg1 on Ca2 + overload The effect of Rg1 on the apoptosis induced by CHX and CTX was detected by Sytox R Green staining and fluorescent microplate reader. Results Rg1 of 10μmol / L and 10μmol / L of Rg1 significantly inhibited the LPS-induced NO and ROS production and phagocytosis of macrophages. Rg1 at 10μmol / L significantly inhibited Ion-induced Ca2 + overload in macrophages and CHX , CTX-induced apoptosis of cells. Conclusion Rg1 has a significant anti-inflammatory effect and can resist apoptosis induced by many factors, providing the basis for further development of immunotherapy drugs.