[Objective] To study the variation of the polysaccharide content of domestic high quality Ophiopogon japonicus tubers at different developmental stages, to determine the quality characteristics and to provide a scientific basis for the GAP quality control and integrated resource development and utilization of O. japonicus. [ Method] Plant tubers collected from Hangzhou O. japonicus and Sichuan Ophiopogon japonicus were graded in accordance with the fresh tuber grading standards. Through microwave kill enzyme -50 ℃ and constant temperature drying, the polysaccharide content of O. japonicus tubers was determined by phenol-sulfuric acid colorimetric method. The law of correlation between the average biomass and the polysaccharide content of tubers at different developmental stages was analyzed using SPSS17.0 statistical software. [Result] The polysaccharide content of Hangzhou O. japonicus and Sichuan O. japonicus tubers at different developmental stages ranged from 24.36% to 41.09% and from 21.54% to 40.29%, respectively. With the increase of developmental stage, the accumulation of photosynthetic products was significantly positively related to the polysaccharide content of O. japonicus tubers (P<0.001). The regression equation was written as y1=7.496ln(x) + 36.45 (R2=0.993) for Hangzhou O. japonicus, and y2=12.68x+19.22 (R2=0.997) for Sichuan O. japonicus. [Conclusion] the accumulation of photosynthetic products in O. japonicus tubers at different developmental stages is closely related to the content of secondary metabolites polysaccharide. It is scientific to divide the domestic high quality O. japonicus tubers by the traditional grade specifications. [Objective] To study the variation of the polysaccharide content of domestic high quality Ophiopogon japonicus tubers at different developmental stages, to determine the quality characteristics and to provide a scientific basis for the GAP quality control and integrated resource development and utilization of O. japonicus. [Method] Plant tubers collected from Hangzhou O. japonicus and Sichuan Ophiopogon japonicus were graded in accordance with the fresh tuber grading standards. Through microwave kill enzyme -50 ° C and constant temperature drying, the polysaccharide content of O. japonicus tubers was determined by phenol -sulfuric acid colorimetric method. The law of correlation between the average biomass and the polysaccharide content of tubers at different developmental stages was analyzed using SPSS 17.0 statistical software. [Result] The polysaccharide content of Hangzhou O. japonicus and Sichuan O. japonicus tubers at different developmental stages ranged from 24.36% to 41.09% and From the increase of developmental stage, the accumulation of photosynthetic products was significantly related to the polysaccharide content of O. japonicus tubers (P <0.001). The regression equation was written as y1 = 7.496ln (x) +36.45 (R2 = 0.993) for Hangzhou O. japonicus, and y2 = 12.68x + 19.22 (R2 = 0.997) for Sichuan O. japonicus. The accumulation of photosynthetic products in O. japonicus tubers at different developmental stages is closely related to the content of secondary metabolites polysaccharide. It is scientific to divide the domestic high quality O. japonicus tubers by the traditional grade specifications.