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目的:探究三七地上茎积累花色苷对其干旱胁迫下抗氧化能力的效应。方法:研究了PEG6000模拟干旱胁迫下三七一年生紫、绿地上茎植株叶可溶性蛋白质和丙二醛(MDA)含量及抗氧化酶比活力。结果:在PEG6000模拟干旱胁迫下,紫、绿地上茎植株叶可溶性蛋白质含量、过氧化氢酶(CAT)和过氧化物酶(POD)比活力及MDA含量均增加、超氧化物歧化酶(SOD)比活力均下降,且绿茎植株叶可溶性蛋白质和MDA含量及紫茎植株叶CAT和POD比活力的升幅、绿茎植株叶SOD比活力的降幅均更大;PEG6000处理结束时,紫茎植株叶的可溶性蛋白质和MDA含量、CAT和SOD比活力均高于绿茎植株叶的,而紫、绿茎植株叶POD的比活力仍几乎相等;紫、绿茎植株叶可溶性蛋白质和MDA含量间的差异均仅达到显著水平,而CAT、POD和SOD比活力间的差异均未达到显著水平。结论:一年生三七地上茎积累花色苷利于其叶可溶性蛋白质含量、CAT和POD比活力在干旱胁迫中的快速上升、滞缓干旱胁迫中SOD比活力下降和MDA含量的上升,并利于胁迫结束时叶高可溶性蛋白质含量、CAT和SOD比活力水平的维持。
Objective: To investigate the effects of anthocyanins accumulated in shoots of aboveground roots of Panax notoginseng against the oxidative stress under drought stress. Methods: The contents of soluble protein and malondialdehyde (MDA) and the activities of antioxidant enzymes in the stems of purple-green and green stems of three-year-old perennials under PEG6000 simulated drought stress were studied. Results: Under PEG6000 simulated drought stress, the content of soluble protein, the activities of catalase (CAT) and peroxidase (POD) and the contents of MDA in the stems of purple and green plants increased, and the activities of superoxide dismutase (SOD) Specific activity and the content of soluble protein and MDA in the leaves of green stems and the increase of specific activities of CAT and POD in the stems and leaves of the stems of green stems, The soluble protein and MDA content, CAT and SOD activities were higher than that of the leaves of green stems, while the specific activities of POD in leaves of purple and green stems were still almost the same. The differences of soluble protein and MDA content between purple and green stems All reached the significant level, while the differences in the specific activities of CAT, POD and SOD did not reach significant levels. CONCLUSION: The annual accumulation of anthocyanins in aerial parts of Panax notoginseng is beneficial to the content of soluble protein, the specific activity of CAT and POD is rapidly increased in drought stress, the SOD activity is decreased and the content of MDA is increased in delayed drought stress, High soluble protein content, CAT and SOD specific activity levels were maintained.