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采用求偶时间观察及性信息素的室内定量生物测定法，确定了华北大黑鳃金龟释放性信息的高峰期为２０：００～２１：００时，活性粗提物的制取应在此时进行。性信息素粗提物的生物测定结果表明：用乙醇浸提性信息素腺体，所得粗提物显示较强引诱活性。色谱分离和收集馏分的生物测定结果表明：组分Ａ具有明显生物活性，是华北大黑鳃金龟性信息素的主要有效成分之一。
The indoor quantitative bioassay of courtship observation and sex pheromone was used to determine the peak of the release information of M. chinensis from 20:00 to 21:00 and the preparation of the active crude extract should be carried out at this time . The bioassay results of the sex pheromone crude extract showed that the crude extract obtained by ethanol extraction of the pheromone gland showed stronger attractant activity. The bioassay results of chromatographic separation and fraction collection showed that component A has obvious biological activity and is one of the main active components of the sex pheromone of the giant sclerotium chinense.