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通过对胶东卫矛不同外植体来源、不同激素种类以及激素配比的比较,初步建立了胶东卫矛组织培养及再生体系:胶东卫矛茎段愈伤组织诱导培养基为MS+6-BA 0.5 mg/L(毫克/升)+IBA 0.05 mg/L(毫克/升),分化培养基为MS+6-BA 0.5 mg/L(毫克/升)+IBA 0.05 mg/L(毫克/升),生根培养基为MS+NAA 0.4 mg/L(毫克/升)+IBA 0.1 mg/L(毫克/升);在此基础上进一步进行了抗生素敏感性实验,确定了胶东卫矛茎段转化的卡那霉素筛选浓度为50 mg/L(毫克/升),羧苄青霉素浓度为200 mg/L(毫克/升),为胶东卫矛的遗传转化奠定了基础。
Through the comparison of different explants sources, different hormone types and hormone proportion of Jiaodong Weiyao, the tissue culture and regeneration system of Jiaodongweingsu was preliminarily established: the callus induction medium of MSW was MS + 6-BA 0.5 mg / L + IBA 0.05 mg / L in medium with MS + 6-BA 0.5 mg / L + IBA 0.05 mg / L (mg / , Rooting medium MS + NAA 0.4 mg / L + IBA 0.1 mg / L (mg / L); on this basis, further antibiotic susceptibility experiments to determine the transformation of stem The screening concentration of kanamycin was 50 mg / L and the concentration of carbenicillin was 200 mg / L, which lays a foundation for the genetic transformation of Jiao-Dong.