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已往研究证实小儿乙型肝炎病毒相关性肾炎 (HBV-GN)的肾组织中存在HBV DNA,而非HBV- GN患儿的肾组织有否存在呢?为此,我们对HBV-GN 及非HBV-GN的小儿肾脏病肾组织同期地进行HBV DNA的检测。 一、材料与方法 临床诊断为HBV-GN 14例,非 HBV-GN 24例.其中肾病综合征9例,血尿待查6例, 肾小球肾炎6例,IgA肾病2例.紫癜性肾炎1例。病理 类型膜性肾炎11例,系膜增生性肾炎11例.膜增生性 肾炎5例,轻微病变5例,其他分别为局灶增生性肾炎 3例,弥漫增生性肾炎2例,1例未见肾小球。 我们应用微量Southern印迹杂交法检测各种小 儿肾脏病的肾组织中HBV DNA。该法优点为可观察 DNA的存在状态,排除血污染可能,且采样不多,适合
Previous studies have confirmed the presence of HBV DNA in the kidneys of children with hepatitis B virus-related nephritis (HBV-GN), but does it exist in kidney tissue of non-HBV-GN children? For this reason, -GN Kidney Disease Kidney disease in the same period for the detection of HBV DNA. Materials and Methods Clinical diagnosis of HBV-GN in 14 cases, non-HBV-GN 24 cases, including nephrotic syndrome in 9 cases, hematuria in 6 cases, glomerulonephritis in 6 cases, IgA nephropathy in 2. Purpura nephritis 1 example. Pathological type membranous nephritis in 11 cases, mesangial proliferative glomerulonephritis in 11 cases. Membranous proliferative glomerulonephritis in 5 cases, 5 cases of minor lesions, the other were focal proliferative glomerulonephritis in 3 cases, diffuse proliferative glomerulonephritis in 2 cases, 1 case not seen Glomerular. We applied trace Southern blot hybridization to detect HBV DNA in renal tissues of various pediatric kidney disease. Advantages of the law for the presence of DNA can be observed, excluding blood contamination may be, and the sample is not small, suitable