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目的探讨系统性红斑狼疮(SLE)患者外周血白细胞中抗粘病毒1(MX1)基因和2′5′-寡腺苷酸合成酶(OAS)1基因的实时定量表达水平与SLE临床表现及病情活动度的相关性。方法收集50例SLE患者,20例非SLE其他自身免疫性疾病患者,25例健康对照人群的临床资料,取外周血抽提总RNA并逆转录成cDNA,运用SYBR green dye I实时定量聚合酶链反应(QT-PCR)在ABI PRISM 7000基因测序仪上检测患者和对照组的MX1和OAS1定量表达水平(以ΔCt值表示)的差异,并与各临床指标及病情活动度进行相关性分析。结果①SLE患者总体的MX1 mRNAΔCt值(3.55±1.39)高于非SLE患者组(2.31±0.52,P=0.000)和正常人(2.23±1.05,P=0.000)。②SLE患者组的OAS1 mRNAΔCt值(4.45±1.56)高于非SLE患者组(3.03±0.76,P=0.000)和正常人(2.75±0.64,P=0.000)。③SLE患者组的OAS1 mRNAΔCT值与SLEDAI积分之间有相关性(r=0.338,P=0.019),与血浆IgA水平有相关性(r=0.475,P=0.001)④SLE患者组的MX1 mRNAΔCT值与SLEDAI积分之间无相关性(r=0.064,P=0.661),与甘油三脂(TG)、胆固醇(TC)、低密度脂蛋白(LDL)2、4 h尿蛋白均有相关性(r=0.428,P=0.003;r=0.383,P=0.009;r=0.394,P=0.007;r=0.316,P=0.025)。⑤在有关节炎的SLE患者中,其MX1和OAS1表达水平升高更明显(3.04±1.42,P=0.004;3.89±1.49,P=0.006)。结论MX1与OAS1在SLE患者中均有表达上调现象,OAS1 mRNA实时表达定量水平对SLE患者的病情活动度判断有意义。二者作为Ⅰ型干扰素诱导表达的基因在SLE发病中均有各自作用。
Objective To investigate the real-time quantitative expression of anti-myxovirus 1 (MX1) gene and 2’5’-oligoadenylate synthase (OAS) 1 gene in peripheral leukocytes of patients with systemic lupus erythematosus (SLE) and the clinical manifestations and condition of SLE Relevance of activity. Methods The clinical data of 50 patients with SLE, 20 patients with non-SLE autoimmune diseases and 25 healthy control subjects were collected. Total RNA was extracted from peripheral blood and reverse transcribed into cDNA. Real-time PCR was performed using SYBR green dye I Response (QT-PCR) The differences in MX1 and OAS1 quantitative expression levels (expressed as ΔCt values) between patients and controls were measured on an ABI PRISM 7000 sequencer and correlated with clinical variables and disease activity. Results ① The overall MX1 mRNAΔCt value in patients with SLE (3.55 ± 1.39) was significantly higher than that in non-SLE patients (2.31 ± 0.52, P = 0.000) and normal subjects (2.23 ± 1.05, P = 0.000). ② The OAS1 mRNAΔCt value in patients with SLE (4.45 ± 1.56) was significantly higher than that in non-SLE patients (3.03 ± 0.76, P = 0.000) and normal subjects (2.75 ± 0.64, P = 0.000). (3) There was a significant correlation between OAS1 mRNAΔCT value and SLEDAI score in patients with SLE (r = 0.338, P = 0.019) and plasma IgA level (r = 0.475, P = 0.001) (R = 0.064, P = 0.661), and urine protein of 2,4 h of triglyceride (TG), cholesterol (TC) and low density lipoprotein (LDL) , P = 0.003; r = 0.383, P = 0.009; r = 0.394, P = 0.007; r = 0.316, P = 0.025). (5) The expression of MX1 and OAS1 in SLE patients with arthritis increased more significantly (3.04 ± 1.42, P = 0.004; 3.89 ± 1.49, P = 0.006). Conclusion Both MX1 and OAS1 are upregulated in SLE patients. The quantitative real-time expression of OAS1 mRNA is of significance in judging the activity of SLE patients. The two genes that are induced as type I interferon have their respective roles in the pathogenesis of SLE.