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①目的探讨低温冻除子宫内膜的适宜参数和临床应用的安全范围。②方法２５只家兔随机分为３组，分别进行活体肌肉冻结实验、肌肉破坏实验和冻后免疫指标检测实验。另选择５例子宫肌瘤合并出血病人，于冷冻后７ｄ手术切除子宫，行组织病理学检查。③结果－４０～－８０℃的温度即足以破坏适宜深度的动物肌肉层，并可调整机体的免疫机能；使连体子宫内膜深达浅肌层凝固坏死。④结论－４０～－８０℃为临床进行冻除子宫内膜的适宜温度。
Objective To investigate the suitable parameters and the safe range of clinical application of cryopreservation of endometrium. Methods Twenty-five rabbits were randomly divided into three groups: live muscle freeze test, muscle destruction test and immune index test after freezing. Another 5 cases of uterine fibroids combined bleeding patients, 7 days after cryopreservation of the uterus, histopathological examination. ③ Results -40 ~ -80 ℃ temperature that is enough to destroy the appropriate depth of the animal muscle layer, and can adjust the body’s immune function; so that the piece of endometrial solidification of superficial myometrial necrosis. ④ Conclusion -40 ~ -80 ℃ for clinical endometrial freezing appropriate temperature.