引进运筹学中的分支定界法用于解析蛋白质及多肽的质谱图，考查了碎片离子质量数、质谱仪的分辨率与可行解数的关系：质量数越小，质谱分辨率越高，可行解数越少，获得的结果也比较确定；反之，则结果的可靠性越小。在此基础上，实际解析了一多肽质谱图，获得了较为满意的结果。运用本法解析有关蛋白质及多肽的质谱图，具有快速、准确、简便的特点，它为生化分析工作者检测蛋白质及多肽的氨基酸序列提供了一条比较可行的途径。 The introduction of branch-and-bound method in operation research was used to analyze the mass spectra of proteins and peptides. The relationship between the mass fraction of fragments and the resolution of mass spectrometer and the number of feasible solutions was investigated. The smaller the mass, the higher the mass spectral resolution, The fewer the results obtained are more certain; the other hand, the less reliable the result is. On this basis, a peptide mass spectrum was actually analyzed and satisfactory results were obtained. Using this method to resolve the mass spectra of proteins and peptides is fast, accurate and easy. It provides a more viable approach for biochemical analysis workers to detect the amino acid sequences of proteins and peptides.