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在植物病原微生物和植物病毒的理论工作及实际工作中,经常遇到两个迫切的问题:如何能够以最迅速同时又最正确的方法,来诊断植物病害的病原,以便确定病害的性质;如何能够可靠地鉴别植物病原微生物间的亲缘关系。关于这两个问题的解决,以前而且现在仍然占据统治地位的是以病状、病原微生物的生物化学、培养等特性作根据的方法。显然这些特性由于不同环境因素的影响,变异度极大,因之常常得不出可靠的结果,最后仍需要依赖手续极度复杂的,条件要求极为严格的接种试验,间接求证.为了迅速并正确的解决这两个问题,苏联的植病工作者,在最近期间,研究出一种以抗原与抗体相互作用作基础的血清学方法(СЕРОЛОГИЛЕЕКИЕМЕТОДПКЧЫ).这个方法在植物病理学中,不论在理论上或实际运用上解决了很多的重要问题.当然这个方法作为植物病理学内的一个普遍法则,还有很多问题需要进一步研究,但毫无疑问的,像在医学上所表现的一样,在植物病理学中,将同样地具有极丰富、极辽阔的发展远景.植物病理学中血清诊断滴定法,是苏联著名植物学家都宁(М.С.ДУНИН)教授及其助手们,在长期实际运用的经验基础上,概括、精炼而成的一项创作,不但适用于教学,而且也是目前植物病理学中一项先进技术,我们要创造性的来运用它,掌握它!
In both theoretical and practical work on phytopathogenic microorganisms and plant viruses, two pressing issues are often encountered: how can the pathogen of plant diseases be diagnosed in the most rapid and simultaneous manner in order to determine the nature of the disease; It is possible to reliably identify the phylogenetic relationships among phytopathogenic microorganisms. The solution to these two problems, formerly and still in dominance, is based on the symptoms, the biochemistry of pathogenic microorganisms, and the culture. Obviously these characteristics due to different environmental factors, the great degree of variability, and often can not get reliable results, and finally still need to rely on extremely complex procedures, the conditions of the very strict vaccination test, indirect verification. In order to quickly and correctly In addressing these two issues, in the recent period, plant-disease workers in the Soviet Union have developed a serological method based on the interaction of antigens with antibodies (СЕРОЛОГИЛЕЕКИЕМЕТОДПКЧЫ) .In this case, this method is used in plant pathology, either theoretically or There are many important problems that have been solved in practice, but as a general rule in plant pathology, there are still many problems that need further study, but there is no doubt that, as demonstrated in medicine, in plant pathology , Will equally have a very rich, very broad prospects for development. Serological diagnostic titration in plant pathology, is the Soviet Union famous botanist Du Ning (М.С.ДУНИН) professors and their assistants, in the long-term practical experience Based on, summarized, refined from a creation, not only for teaching, but also a plant pathology Advanced technology, we need creative ways to use it, master it!