论文部分内容阅读
方法：食物中ＶＫ１、ＶＫ２用己烷提取，以石油醚乙醚（８５１５）为展开剂，于ＧＦ２５４ｎｍ硅胶薄板上纯化，正己烷萃取，离心，８０℃水浴蒸干。用甲醇溶解残留物，经ＯＤＳ－Ｃ１８反相柱，以甲醇为流动相。结果：在１．５ｍｌ／ｍｉｎ的流速下，选择２５４ｎｍ检测，ＶＫ１、ＶＫ２能很好地分离，ＶＫ１和ＶＫ２两者的回收率分别为８８．３％～９０．８％和８９．８％～９１．５％，变异系数为４．８２％～５．４９％。结论：反相液相色谱法简单、准确、适合于食物中ＶＫ１、ＶＫ２的分析。
Methods: VK1 and VK2 in food were extracted with hexane and petroleum ether (8515) as developing solvent, purified on GF254nm silica gel plate, extracted with n-hexane, centrifuged and evaporated in a water bath at 80 ℃. The residue was dissolved in methanol, the ODS-C18 reverse phase column to methanol as the mobile phase. Results: With the flow rate of 1.5ml / min, VK1 and VK2 could be separated well at 254nm. The recoveries of VK1 and VK2 were 88.3% ~ 90.8% and 89.8% ~ 91.5%, coefficient of variation of 4.82% ~ 5.49%. Conclusion: RP-HPLC is simple and accurate and is suitable for the analysis of VK1 and VK2 in food.