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目的克隆斑马鱼前列腺素E合酶3a(prostaglandin E synthase 3a,ptges3a)基因,研究其在斑马鱼胚胎早期发育中的表达情况。方法提取斑马鱼胚胎的总RNA,制备DIG标记的ptges3a RNA反义探针,整胚原位杂交研究ptges3a在斑马鱼胚胎早期发育过程的表达。结果 ptges3a在shield期前普遍性表达,10体节期在后脑神经龙骨处有特异性表达,18体节期在后脑有特异性表达,在26 hpf时期头部表达较多,并且在肾小管有特异性表达,36 hpf ptges3a在头部较高表达。结论 ptges3a可能参与了脑部发育和肾小管形成。
Objective To clone the gene of zebrafish prostaglandin E synthase 3a (ptges3a) and study its expression in the early development of zebrafish embryos. Methods The total RNA was extracted from zebrafish embryos and the antisense probe of pIGs3a RNA was prepared. The expression of ptges3a in early embryonic development of zebrafish was studied by in situ hybridization. Results ptges3a was universally expressed in the early stage of the shield. The expression of ptges3a was specific in the keel of the posterior segment of the brain during the 10-day period. The expression of ptges3a was specific in the posterior segment of the brain at the 18th day, and more in the head during 26 hpf. Specific expression of 36 hpf ptges3a was higher in the head. Conclusion ptges3a may be involved in brain development and tubular formation.