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目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体TRAILR2、甲胎蛋白AFP和核因子NFκBp65在肝细胞癌(hepatocellularcarcinoma,HCC)凋亡中的作用及相互关系。方法采用免疫组织化学方法检测TRAILR2、AFP、NFκBp65在40例HCC及12例正常肝组织中的表达变化。结果TRAILR2和NFκBp65在40例HCC和12例正常肝组织中均表达,但是HCC组的表达明显增强,两组间表达具有显著性差异(P<0.01);40例HCC中有7例不表达AFP,其余33例可见明显表达,正常肝组织组不表达AFP,两组间表达差异有显著性(P<0.01);HCC中TRAILR2的表达与AFP的表达呈负相关(r=0.464,P<0.01),与NFκBp65的表达也呈负相关(r=0.648,P<0.01)。结论TRAILR2、AFP、NFκBp65与HCC的凋亡有关,AFP抑制BCC凋亡与TRAILR2表达下调有关,AFP下调TRAILR2表达可能是通过NFκBp65的间接作用。
Objective To investigate the roles and relationships of TRAILR2, AFP and NFκBp65 in the apoptosis of hepatocellular carcinoma (HCC). Methods The expressions of TRAILR2, AFP and NFκBp65 in 40 HCC and 12 normal liver tissues were detected by immunohistochemistry. Results TRAILR2 and NFκBp65 were both expressed in 40 HCC cases and 12 normal liver tissues, but the expression of TRAILR2 and NFκBp65 were significantly increased in HCC group (P <0.01), and 7 of 40 HCC cases did not express AFP (P <0.01). There was a negative correlation between TRAILR2 expression and AFP expression in HCC (r = 0.464, P <0.01) ), But also negatively correlated with the expression of NFκBp65 (r = 0.648, P <0.01). Conclusion TRAILR2, AFP and NFκBp65 are related to the apoptosis of HCC. AFP inhibited the apoptosis of BCC and the expression of TRAILR2 was down-regulated. The down-regulation of TRAILR2 expression by AFP may be mediated indirectly by NFκBp65.