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在过去十年中,植物细胞培养已成为基础研究和应用研究的辅助方法。对这些研究的进一步发展,突变体的应用有时具有决定性的作用。因为:1.突变体是研究遗传结构的强有力工具。2.突变体易于进行植物新陈代谢途径的研究。3.某些突变可作为筛选体细胞杂种及进行叶绿体、线粒体、细胞核转移和染色体分离等遗传研究时选择的标志。4.从植物细胞和原生质培养选出的突变体可用于提高植物育种的遗传变异性。用植物细胞培养方法分离突变体有不少优点,如在相当小的范围内即可处理并检测大量细胞,甚至可以获得频率低
In the past decade, plant cell culture has become an adjunct to basic research and applied research. For the further development of these studies, the use of mutants is sometimes decisive. Because: 1. Mutants are powerful tools for studying genetic structure. 2. Mutants are easy to study plant metabolic pathways. Some mutations can be used as screening markers for somatic hybrids and genetic selection of chloroplast, mitochondria, nuclear transfer and chromosome segregation. 4. Mutants selected from plant cell and protoplasm cultures can be used to increase the genetic variability of plant breeding. Isolation of mutants using plant cell culture has many advantages, such as the ability to process and detect large numbers of cells over a relatively small range and even achieve low frequency