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实验采用离体脑片全细胞膜片箝记录方法 ,观察了细胞因子白介素 1β(IL 1β)和IL 2对大鼠离体脑片视上核神经元膜电位及自发放电的影响 ,以期探明免疫应答大鼠视上核神经元对细胞因子敏感性的变化。结果显示 ,用 10 0U/mlIL 1β灌流脑片 ,正常对照的 (n =15 )和脂多糖 (lipopolysaccharideLPS)腹腔注射 9d的大鼠视上核神经元 (n =2 0 )超极化 ,同时伴有自发放电频率的下降 ;应用 10 0U/mlIL 2 ,大部分正常对照视上核神经元 (n =14)表现为超极化 ,自发放电减少 ,剩余部分 (n =3)变化不明显 ;在LPS免疫 9d大鼠离体脑片上的 45个视上核神经元中 ,10 0U/ml的IL 2使其中 19个表现为去极化并伴有自发放电频率增加 ,16个变化不明显 ,其余 10个表现为超极化伴放电频率下降。以上结果表明 ,在免疫应答中 ,视上核神经元对细胞因子IL 2的敏感性 ,在一定的程度上发生了改变 ,细胞因子IL 2可能参与了视上核神经元的功能调节 ,进而在免疫应答过程中发挥了调节作用。
In vitro experiments were performed to observe the effects of interleukin-1β (IL-1β) and IL-2 on membrane potential and spontaneous firing of supraoptic nucleus neurons in isolated rat brains. Responses to suprabasal suprabasal neurons to cytokine sensitivity changes. The results showed that the supraoptic nucleus neurons (n = 20) were hyperpolarized in the normal control group (n = 15) and lipopolysaccharide LPS group 9 days after perfused with 100 U / ml IL-1β There was a decrease in the frequency of spontaneous discharge. When 10 μU / ml IL 2 was applied, most of the normal supraoptic nucleus neurons (n = 14) showed hyperpolarization and decreased spontaneous discharge with no significant change in the remaining part (n = 3) Among the 45 supraoptic neurons on the isolated rat brain slices from LPS-immunized mice, 19 of them showed depolarization accompanied by an increase of spontaneous discharge frequency and no obvious change of 16, 10 showed a decrease in the frequency of hyperpolarization with discharge. The above results show that in the immune response, supranuclear neurons on the cytokine IL 2 sensitivity has changed to some extent, the cytokine IL 2 may be involved in the functional regulation of supranuclear neurons, and then in the The immune response plays a regulatory role.