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目的:用表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)分析肝内胆管癌和健康者血清蛋白指纹图谱的变化,建立能鉴别肝内胆管癌和健康者血清标志物的诊断模型。方法:收集33例肝内胆管癌和31例健康者的血清,用SELDI-TOF-MS及配套H50(疏水芯片)蛋白质芯片检测蛋白指纹的表达。用Biomarker Patterns Software分析软件进行数据处理,建立区分肝内胆管癌和健康者的蛋白质指纹图谱表达模型。结果:在分子量2000~20000范围内,共检测到127个有差异的蛋白峰,其中10个差异有显著性(P<0.01),建立了由2个差异蛋白组成的肝内胆管癌诊断模型,其诊断灵敏度为96.9%(32/33),特异性为96.7(30/31)。结论:用SELDI-TOF-MS技术初步建立的区分肝内胆管癌和健康者的蛋白指纹表达模型可以区分肝内胆管癌和健康者,可能为肝内胆管癌的诊断提供新的手段。
OBJECTIVE: To analyze the changes of serum protein fingerprints in patients with intrahepatic cholangiocarcinoma and healthy persons by using surface-enhanced laser desorption / ionization time of flight mass spectrometry (SELDI-TOF-MS) to establish a diagnostic model that can identify serum markers of intrahepatic cholangiocarcinoma and healthy people . Methods: Sera from 33 patients with intrahepatic cholangiocarcinoma and 31 healthy controls were collected. Protein fingerprinting was detected by SELDI-TOF-MS and H50 (hydrophobic chip) protein chip. Biomarker Patterns Software analysis software for data processing, the establishment of a distinction between intrahepatic cholangiocarcinoma and healthy protein fingerprinting expression model. Results: A total of 127 differential proteins were detected in the range of 2000-20000, of which 10 were significant (P <0.01). A diagnostic model of intrahepatic cholangiocarcinoma consisting of two differential proteins was established, The diagnostic sensitivity was 96.9% (32/33) and the specificity was 96.7 (30/31). CONCLUSIONS: The protein fingerprinting model that distinguishes intrahepatic cholangiocarcinoma from healthy cholangitis patients initially established by SELDI-TOF-MS technique can distinguish intrahepatic cholangiocarcinoma from healthy controls and may provide a new method for the diagnosis of intrahepatic cholangiocarcinoma.