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近年来辣椒病毒病在辽宁省各地发生较为普遍。为了弄清毒原种群,几年来,在我组鉴定出主要毒原为烟草花叶病毒(TMV)和黄瓜花叶病毒(CMY)的同时,又在从沈阳地区采集的21份标样中,鉴定出分离物6号,系烟草蚀纹病毒(TEV),这是在辣椒花叶病中检测出的一个新的毒原。辣椒分离物6号在以下的指示植物上,经摩擦接种后,叶面表现为:在辣椒上叶片呈黄化斑驳,心叶稍细长,有时叶肉并有微细蚀纹斑,植株略矮。在心叶烟上表现斑驳花叶,在普通烟(黄苗榆)叶上现蚀纹。在昆诺藜叶上生局部枯斑。在曼陀萝叶上生明脉和花叶。在百日草上生花叶症。在千日红和马铃薯上无症。采用其他原抗血清反证的方法,进行血清学反应测定,它对TMV、CMV、PVX、PVY的抗血清均无沉淀反应。采用辣椒6号分离物病叶,以叶浸蘸法,作电镜观察,病毒粒体为线状,长约730nm。病毒汁液体外三常规测定,其钝化温度约为55℃(10分钟);稀释限点为10~(-4);体外存活期5—6日(20℃)。因此初步认为它是侵染辣椒的烟草蚀纹病毒(TEV)。
In recent years pepper virus disease occurs more commonly in Liaoning Province. In order to understand the virulent population, in the past few years, while identifying the major virulent strains of TMV and CMY in our group, among the 21 samples collected from Shenyang, Isolate No. 6 was identified as a TEV, a new toxin detected in pepper mosaic disease. Chilli isolate No. 6 on the following indications of plants, after inoculation by friction, the foliage showed: yellow leaves mottled mottled leaves, slightly slender heart leaf, leaf and sometimes slightly etched plaque, the plant slightly shorter. In the heart leaf mottled mottled mosaic, in ordinary tobacco (yellow elm) leaf erosion patterns. Health quinoa leaves on the local spot. In the mandala leaves on the Ming veins and mosaic. Mosaic on the zinnia. Asymptomatic on Amaranth and Potato. Using other anti-anti-prolactin method, serological reaction determination, it has no precipitation reaction against TMV, CMV, PVX, PVY antiserum. Pepper 6 leaves were used to digest the leaves of the pepper, dipped in leaf dipping method for electron microscopy, the virus mitochondria for the linear, about 730nm. Viral juice three routine determination of the body, the passivation temperature of about 55 ℃ (10 minutes); dilution limit of 10 -4; in vitro survival 5-6 days (20 ℃). It is therefore preliminarily considered as a tobacco etch virus (TEV) that infects peppers.