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目的 :研究不同剂量光动力作用细胞内游离钙([Ｃａ2 +]ｉ)的变化 ,探讨钙超载在光动力诱导Ｃ6胶质瘤细胞坏死和凋亡过程中的作用。方法 :常规培养Ｃ6胶质瘤细胞 ,以不同光动力剂量处理对数生长期的实验组细胞后 ,应用Ｆｌｕｏ 3 ＡＭ为细胞内钙离子荧光指示剂 ,激光共聚焦显微
Objective: To study the changes of intracellular free calcium ([Ca2 +] i) under different doses of photodynamic therapy and to explore the role of calcium overload in the process of photodynamic therapy of C6 glioma cells. Methods: C6 glioma cells were cultured routinely and treated with different doses of photodynamic therapy in logarithmic growth phase. Fluo 3 AM was used as intracellular calcium ion fluorescent indicator. Confocal laser scanning microscopy