目的:建立高效、灵敏、快速的人血浆中百草枯的HPLC血药浓度测定方法。方法:以5-溴脲嘧啶为内标物,血浆经10%三氯乙酸甲醇去蛋白。采用XBridge-C18柱,流动相为乙腈-缓冲盐(V∶V,5∶95),缓冲盐为25.0 mmol/L磷酸二氢钠,含1.0 mmol/L十二烷基硫酸钠,pH 2.6,流速1.0 ml/min,检测波长258 nm,柱温35℃。结果:百草枯和内标的出峰时间分别为6.0 min和3.5 min,百草枯在0.05μg/ml～10μg/ml范围内线性关系良好(R2=0.9998)。低、中、高3种浓度的日内、日间精密度RSD均<6%,相对回收率100.623±5.443%,平均绝对回收率88.374±5.669%。结论:本方法操作简便易行,准确率高,可作为快速、准确测定人血浆中百草枯血药浓度的有效方法,为临床诊断提供依据。 Objective: To establish an HPLC method for the determination of paraquat in human plasma with high efficiency, sensitivity and rapidity. Methods: Using 5-bromouracil as internal standard, the plasma was deproteinized with 10% trichloroacetic acid methanol. The mobile phase consisted of acetonitrile-buffered saline (V:V, 5:95) with a buffer salt of 25.0 mmol / L sodium dihydrogenphosphate, containing 1.0 mmol / L sodium lauryl sulphate, pH 2.6, Flow rate 1.0 ml / min, detection wavelength 258 nm, column temperature 35 ℃. Results: The peak time of paraquat and internal standard was 6.0 min and 3.5 min respectively. The linearity of paraquat in the range of 0.05 μg / ml ~ 10 μg / ml was good (R2 = 0.9998). RSD of intra-day and inter-day precision of low, medium and high concentration were less than 6%, the relative recovery was 100.623 ± 5.443%, and the average absolute recovery was 88.374 ± 5.669%. Conclusion: The method is simple and easy to operate, with high accuracy. It can be used as an effective and rapid method for rapid and accurate determination of paraquat in human plasma and provide the basis for clinical diagnosis.