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采用旋转薄膜蒸发法结合挤压过滤工艺制备大豆磷脂脂质体,所制得脂质体的平均粒径为217 nm,跨距0.838,负染色脂质体经透射电镜观察,呈明显的双层膜圆球型结构。以胆酸钠盐和脱氧胆酸钠盐研究其与脂质体之间的相互作用,将500 nm波长处的可见光吸收值评价胆盐-脂质体混悬液的浊度,测定脂质体的粒径变化情况。在胆酸盐加入的初期,由于胆酸盐和脂质体形成混合胶团,致使脂质体的粒径和浊度值稍有增加,进一步加入的胆酸盐使脂质体的粒径和浊度值下降。胆盐和脂质体间的相互作用经历了数个重排现象,使脂质体的粒径发生规律性的变化,这与脂质体的体内稳定性密切相关,也可反映脂质体的载药和释药特性。
Soybean phospholipid liposomes were prepared by rotary thin-film evaporation combined with extrusion filtration. The average diameter of liposomes prepared was 217 nm and the span was 0.838. Negative-staining liposomes were observed by transmission electron microscope Membrane spherical structure. The interaction between liposomes and sodium cholic acid and sodium deoxycholate was studied. The turbidity of the bile salt-liposome suspension was evaluated by visible light absorbance at 500 nm. Particle size changes. In the initial period of cholate addition, due to the formation of mixed micelles with cholate and liposome, the size and turbidity value of the liposomes are slightly increased. The further addition of bile salts makes the liposome size and Turbidity drops. The interaction between bile salts and liposomes undergoes several rearrangements, resulting in a regular change in the particle size of the liposomes, which is closely related to the in vivo stability of the liposomes and may also reflect the Drug loading and drug release characteristics.