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目的观察银杏叶片对动脉粥样硬化性大鼠主动脉壁清道夫受体A的表达及血清炎性因子变化的影响,探讨银杏叶片抗动脉粥样硬化斑块形成的新机制。方法 Wistar雄性大鼠45只,随机分成对照组、模型组及银杏叶片组。测定各组大鼠血糖、血脂、血钙、血清C-反应蛋白(C-reactive protein,CRP)、可溶性细胞间黏附分子-1(soluble intercellular adhesion molecule-1,sICAM-1)和可溶性血管细胞黏附分子-1(soluble vascular cell adhesion molecule-1,sVCAM-1)水平,免疫组化法观察清道夫受体A在动脉壁表达的情况,并观察清道夫受体A表达与炎症因子水平的相关性。结果与对照组比较,模型组血糖、血钙明显升高(P<0.05),甘油三酯(TG)、血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平显著升高(P<0.01);新生内膜面积显著增厚,内膜增生百分比显著变大,管腔狭窄指数显著变小,差异均有统计学意义(P<0.01);CRP、sICAM-1、sVCAM-1水平显著升高(P<0.01)。与模型组比较,银杏叶片组血糖、血钙明显降低(P<0.05),TG、TC、LDL-C水平显著降低(P<0.01)。模型组主动脉管腔比银杏叶片组明显狭窄(P<0.05)。清道夫受体A在动脉壁有表达,而银杏叶片组较模型组3项指标显著改善,差异均有统计学意义(P<0.01)。银杏叶片组较模型组可显著降低动脉粥样硬化大鼠血清CRP、sICAM-1、sVCAM-1水平(P<0.01)。血清炎症因子(CRP、sICAM-1、sVCAM-1)水平与动脉壁清道夫受体A阳性表达面积百分比呈正相关(r分别为0.701、0.604、0.581,均P<0.01)。结论动脉粥样硬化大鼠血清中炎症因子水平增高、动脉壁清道夫受体A表达增强,且清道夫受体A表达与炎症因子水平密切相关。银杏叶片可降低炎症因子水平、抑制清道夫受体A表达。
Objective To observe the effect of Ginkgo biloba leaves on the expression of scavenger receptor A and the changes of serum inflammatory factors in the aortic wall of atherosclerotic rats and to explore the new mechanism of ginkgo leaf anti-atherosclerotic plaque formation. Methods 45 male Wistar rats were randomly divided into control group, model group and ginkgo leaf group. Blood glucose, blood lipid, serum calcium, serum C-reactive protein (CRP), soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion The expression of scavenger receptor A in the arterial wall was observed by immunohistochemistry and the correlation between scavenger receptor A expression and inflammatory cytokines . Results Compared with the control group, the levels of blood glucose and serum calcium in the model group were significantly increased (P <0.05) and the levels of triglyceride (TG), serum total cholesterol (TC) and low density lipoprotein cholesterol P <0.01). The neointimal area was significantly thicker, the percentage of intimal hyperplasia was significantly larger, and the stenosis index was significantly smaller (P <0.01). The levels of CRP, sICAM-1 and sVCAM-1 The level was significantly higher (P <0.01). Compared with the model group, the levels of blood glucose and serum calcium in Ginkgo biloba group were significantly decreased (P <0.05), while the levels of TG, TC and LDL-C in Ginkgo biloba group were significantly decreased (P <0.01). The aortic lumen of the model group was significantly stenosed than that of the ginkgo leaf group (P <0.05). Scavenger receptor A expression in the arterial wall, ginkgo leaf group than the model group significantly improved the three indicators, the difference was statistically significant (P <0.01). Compared with the model group, Ginkgo biloba extract significantly reduced the levels of serum CRP, sICAM-1 and sVCAM-1 in atherosclerotic rats (P <0.01). Serum levels of inflammatory cytokines (CRP, sICAM-1, sVCAM-1) were positively correlated with the percentage of scavenger receptor A positive area (r = 0.701,0.604,0.581, all P <0.01). Conclusions The level of inflammatory cytokines in serum of atherosclerotic rats is increased, the expression of scavenger receptor A in the arterial wall is enhanced, and the expression of scavenger receptor A is closely related to the level of inflammatory cytokines. Ginkgo biloba leaves can reduce the level of inflammatory cytokines, scavenger receptor A expression.