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目的:比较不同采收期大花罗布麻叶中总黄酮的含量并评价出最佳采收期。方法:采用超声法提取大花罗布麻叶中的总黄酮,紫外-可见分光光度法于508 nm下测定。结果:芦丁在22.78～113.88 mg.L-1内呈良好的线性关系(r=0.999 8),平均加样回收率为98.87%,RSD为1.03%(n=9);大花罗布麻叶不同采收期的测定结果为:4～5月份相对较低,6～9月份稳步增长,10月份相对9月份有所降低。结论:本方法简便、准确,重现性好,可用于评价大花罗布麻叶的最佳采收期,为该药材合理开发利用提供科学依据。
Objective: To compare the content of total flavonoids in Apocynum leaves of different harvesting stages and to evaluate the best harvest time. Methods: Ultrasound was used to extract the total flavonoids from Apocynum leaves, and UV-Vis spectrophotometry was used to determine the total flavonoids. Results: Routine showed a good linearity (r = 0.999 8) in the range of 22.78-113.88 mg · L-1 with an average recovery of 98.87% and a RSD of 1.03% (n = 9) The results of different harvesting period are: 4 ~ May relatively low, steady growth from June to September, October relative to September decreased. Conclusion: The method is simple, accurate and reproducible. It can be used to evaluate the best harvest time of Apocynum leaves, and provide a scientific basis for the rational development and utilization of Apocynum officinalis.