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【目的】从胰高血糖素样肽-1(GLP-1)合成、分泌和灭活3个环节探索健胰方影响GLP-1改善胰岛细胞功能的可能机制。【方法】采用高脂饲料联合链脲佐菌素(STZ)法诱导糖尿病模型大鼠,随机分为模型组、复方组与正常组,干预4周。快速血糖仪测定血糖,Luminex液相蛋白芯片技术测定血浆GLP-1和胰岛素(insulin)水平,实时荧光定量PCR检测胰腺组织胰岛—十二指肠同源盒基因-1(PDX-1)m RNA表达,免疫组织化学法检测回肠L细胞合成GLP-1,酶联免疫法检测Ⅳ型二肽基肽酶(DPP-Ⅳ)。【结果】健胰方可显著降低糖尿病模型大鼠的空腹及糖负荷后血糖水平(P<0.05),促进糖尿病大鼠血中胰岛素的分泌(P<0.05),提高胰腺PDX-1 m RNA表达(P<0.05),提高血浆GLP-1水平与回肠组织GLP-1阳性反应面积及累积光密度值(P<0.05);但血清DPP-Ⅳ各组间比较无显著性差异(P>0.05)。【结论】健胰方可能是通过调控GLP-1的合成与分泌,从而促进PDX-1基因表达和胰岛素分泌以降低糖尿病大鼠血糖水平的。
【Objective】 To explore the possible mechanism of Jian Pan Fang on GLP-1 in improving islet cell function from three aspects of synthesis, secretion and inactivation of GLP-1. 【Methods】 Diabetic rats were induced by high-fat diet combined with streptozotocin (STZ) and randomly divided into model group, compound group and normal group for 4 weeks. Fasting glucose meter was used to measure the blood glucose, Luminex liquid protein chip technique was used to measure plasma GLP-1 and insulin levels, and pancreatic islet-duodenal homeobox gene 1 (PDX-1) m RNA was detected by real-time fluorescence quantitative PCR The expression of GLP-1 was detected by immunohistochemistry in ileal L cells and the type IV dipeptidyl peptidase (DPP-IV) was detected by enzyme-linked immunosorbent assay. 【Results】 Jianpi Decoction can significantly reduce the fasting blood glucose and blood sugar levels (P <0.05), promote the secretion of insulin (P <0.05) and improve the expression of PDX-1 mRNA in diabetic rats (P <0.05), and increased the plasma GLP-1 level and the GLP-1 positive reaction area and the cumulative optical density (P <0.05). However, there was no significant difference between the serum DPP- . 【Conclusion】 Jian-pan prescription may be through the regulation of GLP-1 synthesis and secretion, thereby promoting PDX-1 gene expression and insulin secretion to reduce blood glucose levels in diabetic rats.