目的采用复合模式介质Capto core 700纯化轮状病毒(rotavirus,RV),去除培养液中的残余细胞宿主蛋白和DNA,提高病毒收率。方法将RV LH9毒种接种Vero细胞,制备RV原液,澄清、超滤后,用Capto core 700纯化:样品LH9、LH9+150 mmol/L Na Cl[以缓冲液A(20 mmol/L PB+150 mmol/L Na Cl,p H 7.0)作为缓冲液]上样纯化分别标记为A、B组合,样品LH9+300 mmol/L Na Cl[以缓冲液B(20mmol/L PB+300 mmol/L Na Cl,p H 7.0)作为缓冲液]上样纯化标记为C组合,样品LH9+450 mmol/L Na Cl[以缓冲液C(20 mmol/L PB+450 mmol/L Na Cl,p H 7.0)作为缓冲液]上样纯化标记为D组合,洗脱,收集流穿峰,检测纯化后病毒的滴度和收率、RV RNA、RV抗原性、残余宿主细胞蛋白(HCP)和DNA;并用初步筛选出的纯化组合纯化3批RV超滤浓缩液,检测各项指标,进行进一步验证。结果用20 mmol/L PB+150 mmol/L Na CL缓冲体系和样品中加入150 mmol/L Na Cl的组合纯化RV,病毒收率在80%以上,病毒核酸完整,其抗原性未发生改变,可去除94%以上的HCP,残余DNA符合《中国药典》三部(2010版)标准。结论 Capto core 700纯化RV收率和残余蛋白去除效率均较高,组合B操作相对简便,工艺时间短,病毒收率高,更适于RV的纯化。 OBJECTIVE To purify rotavirus (RV) with complex pattern medium Capto core 700 to remove residual cellular host protein and DNA in culture solution and improve the yield of virus. Methods The RV LH9 strain was inoculated into Vero cells and the RV stock solution was prepared and clarified. After ultrafiltration, it was purified with Capto core 700: Sample LH9, LH9 + 150 mmol / L NaCl [buffer A (20 mmol / L PB + 150 mmol / L NaCl, p H 7.0) as buffer solution] were labeled as A and B respectively. Samples LH9 + 300 mmol / L NaCl [buffer B (20 mmol / L PB + 300 mmol / L Na Cl, p H 7.0) as buffer] was labeled with a C combination and samples LH9 + 450 mmol / L NaCl [buffer C (20 mmol / L PB + 450 mmol / L NaCl, p H 7.0) As a buffer solution] was labeled with D, elution was performed, and the flow-through peak was collected. The purified virus titer and yield, RV RNA, RV antigenicity, residual host cell protein (HCP) and DNA were determined. The three purified batches of purified RV ultrafiltration concentrate were selected and tested for further validation. Results RV was purified with a combination of 20 mmol / L PB + 150 mmol / L NaCl buffer and 150 mmol / L NaCl. The yield of the virus was above 80%. The viral nucleic acid was intact and its antigenicity was unchanged. Can remove more than 94% of HCP, the residual DNA in line with the “Chinese Pharmacopoeia” three (2010 version) standards. Conclusion Capto core 700 purified RV yield and residual protein removal efficiency are high, the combination of B operation is relatively simple, short process time, high yield of the virus, more suitable for the purification of RV.