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目的:建立HPLC法同时测定康复新液中的尿嘧啶、次黄嘌呤及肌苷含量。方法:色谱柱:Wates SunfireTM C18柱(250 mm×4.6 mm,5μm);甲醇-3%甲醇溶液(含0.07%醋酸)梯度洗脱;检测波长254 nm;进样量:10μl。结果:尿嘧啶在4.92～34.44μg.ml-1(r=0.999 9),次黄嘌呤在12.43～87.06μg.ml-1(r=0.999 9),肌苷在24.77～173.40μg.ml-1(r=0.999 9)的浓度范围内呈良好的线性关系。尿嘧啶的平均回收率为102.63%(RSD=1.78%);次黄嘌呤的平均回收率为99.13%(RSD=1.89%);肌苷的平均回收率为98.98%(RSD=1.33%)。结论:该法操作简便、准确,重复性、稳定性好,可用于同时检测康复新液中尿嘧啶、次黄嘌呤及肌苷的含量。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of uracil, hypoxanthine and inosine in KFF. Methods: Column: Wates SunfireTM C18 column (250 mm × 4.6 mm, 5 μm); gradient elution with methanol-3% methanol (containing 0.07% acetic acid); detection wavelength 254 nm; injection volume: 10 μl. Results: The results showed that uracil at 4.92-34.44μg.ml-1 (r = 0.999 9), hypoxanthine at 12.43-87.06μg.ml-1 (r = 0.999 9) and inosine at 24.77-173.40μg.ml-1 (r = 0.999 9) concentration range showed a good linear relationship. The average recovery of uracil was 102.63% (RSD = 1.78%). The average recovery of hypoxanthine was 99.13% (RSD = 1.89%). The average recovery of inosine was 98.98% (RSD = 1.33%). Conclusion: The method is simple, accurate, reproducible and stable. It can be used for simultaneous determination of uracil, hypoxanthine and inosine in Kangfuxin solution.