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应用逆转录─多聚酶键反应（RT－PCR）检测卵巢癌12例，大肠癌15例，肝癌2例肿瘤多药耐药基因（MDR1）表达。以长春新碱（VCR）敏感细胞株KB和耐药株KBr分别作阴性及阳性对照，卵巢癌患者MDR1表达增高者为50％（6／12），大肠癌增高者75％（10／15）卵巢癌MDR1表达低者化疗效果较好；大肠癌患者MDR1表达高者似与术前局部用化疗药有关。
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of multidrug resistance gene (MDR1) in 12 cases of ovarian cancer, 15 cases of colorectal cancer, and 2 cases of hepatocellular carcinoma. VCR-sensitive cell line KB and drug-resistant KBr were used as negative and positive controls, respectively. The expression of MDR1 in ovarian cancer patients was increased by 50% (6/12), and the increase in colorectal cancer was increased by 75% (10/15). The chemotherapy with low expression of MDR1 in ovarian cancer has better effect; the high expression of MDR1 in patients with colorectal cancer seems to be related to the local use of chemotherapy drugs before surgery.