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目的:动态观察脑卒中后大鼠行为学改变及颐脑解郁方对其早期的干预作用。方法:经Open-Field行为学评分后,将雄性Wistar大鼠随机分为正常组、假手术组、MCI组、中药组、西药组、PSD组、VD组。模型制备采用同种系微栓子体外注入法,由颈外动脉推注0.3ml同种异体血栓栓子;假手术组推注同等剂量生理盐水,并灌胃蒸馏水,中药组及西药组在模型制备后,予颐脑解郁方、尼莫地平干预给药4周;PSD组术后7d予21d慢性不可预知的温和应激加孤养复制PSD模型;VD组术后28d经水迷宫筛选后进入实验。模型复制成功后,分别于第1、2、4周进行穿梭箱主动回避反应实验,记录主动逃避(AAR)的次数、被动逃避(PAR)的次数以及被电击时间,并统计分析。结果:VD组的AAR、PAR次数最低,中药组的AAR、PAR次数均比未治疗组高,两组比较AAR及PAR都有显著性差异(P<0.05,P<0.01)。结论:颐脑解郁方有改善学习和记忆的功能,有利于脑卒中大鼠认知功能的恢复。
OBJECTIVE: To dynamically observe the behavioral changes of rats after stroke and the early intervention of Yinao Jieyu prescription. Methods: After the Open-Field behavioral score, male Wistar rats were randomly divided into normal group, sham operation group, MCI group, TCM group, western medicine group, PSD group and VD group. The model was prepared by in vitro injection of the same kind of micro-emboli, 0.3ml allogeneic thromboembolism was injected from the external carotid artery; the same dose of saline was injected into the sham-operated group and the distilled water was infused into the model group After preparation, the rats were treated with Yinao Jieyu Decoction and Nimodipine for 4 weeks. The PSD model group was treated with mild and unpredictable mild stress 21d after 7d, . After successful replication of the model, active shuttle avoidance experiments were conducted at weeks 1, 2 and 4, and the number of active avoidance (AAR), number of passive avoidance (PAR) and time of shock were recorded and statistically analyzed. Results: AAR and PAR were the lowest in VD group and AAR and PAR in Chinese medicine group were higher than those in untreated group. There was significant difference between AAR and PAR in both groups (P <0.05, P <0.01). Conclusion: Yinao Jieyu decoction has the function of improving learning and memory, which is beneficial to the recovery of cognitive function in stroke rats.