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目的：比较国内外不同厂家色谱柱对人胰岛素注射液的分离效果。方法：以不同配比的硫酸盐缓冲液和５０％乙腈溶液为流动相，检测波长为２１４ｎｍ。结果与结论：通过调整流动相的不同配比，国内外不同厂家色谱柱均可使人胰岛素与脱酰胺胰岛素及防腐剂得到良好分离。
Objective: To compare the separation efficiency of human insulin injection with different manufacturers at home and abroad. Methods: Sulfate buffer with different ratios and 50% acetonitrile as mobile phase, the detection wavelength was 214nm. RESULTS AND CONCLUSION: By adjusting the different ratios of mobile phase, the chromatographic columns of different manufacturers at home and abroad can make the separation of human insulin and deamidated insulin and preservative well.