论文部分内容阅读
目的:研究缺血缺氧新生大鼠海马结构(主要为CA1)磷酸化c-AMP反应元件结合蛋白质(phosphorylatedcylicAMPresponseelementbindingprotein,p-CREB)变化以及GM1对其的影响。方法:7d龄SD仔鼠共108只,随机分为3组:假手术对照组(36只),缺血缺氧组(HI组,36只)及缺血缺氧+神经节苷脂组(GM1组,36只),于模型建立后1,4,12,24,48和72h处死,免疫组化观察海马CA1区p-CREB的变化。结果:对照组各时间点之间p-CREB的表达差异无显著性意义(F=0.48,P>0.05);HI组、GM1组CA1区p-CREB的表达一过性升高后迅速下降,HI组、GM1组与对照组相比有明显的差异(P<0.05);HI组与GM1相比无明显差异(P>0.05)。结论:缺血缺氧后缺血敏感CA1区p-CREB的表达呈现一过性的升高后迅速下降,GM1不能抑制p-CREB的表达,对神经元的存活没有损害作用,具有较大的临床应用潜能。
Objective: To investigate the changes of phosphorylated c-AMP-responsive protein-binding protein (p-CREB) in the hippocampus of hippocampus of neonatal hypoxic-ischemic rats and the effect of GM1 on it. Methods: A total of 108 SD pups aged 7 days were randomly divided into 3 groups: sham-operation control group (36 rats), HI group (36 rats) and ischemia-hypoxia + ganglioside group 36 rats in GM1 group) were sacrificed at 1, 4, 12, 24, 48 and 72 h after model establishment. The expression of p-CREB in hippocampal CA1 area was observed by immunohistochemistry. Results: The expression of p-CREB in the control group was not significantly different at each time point (F = 0.48, P> 0.05) There was significant difference between HI group and GM1 group and control group (P <0.05), but there was no significant difference between HI group and GM1 (P> 0.05). CONCLUSION: The expression of p-CREB in ischemia-reperfusion-sensitive CA1 region is transiently increased after hypoxia-ischemia, but decreased rapidly. GM1 can not inhibit the expression of p-CREB and has no detrimental effect on the survival of neurons. Clinical potential.