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以人的红细胞膜为抗原，采用选择性免疫抑制的程序，免疫BALB／C小鼠。经免疫的小鼠脾细胞与SP2／0骨髓瘤细胞融合，用血凝法筛选出3株分泌抗人红细胞膜种属特异性单克隆抗体的杂交瘤，即M1A7C4．M3A5B7和M3D9F1。连续传代2个月及复苏冻存半年的杂交瘤，仍能稳定地分泌抗人红细胞膜单克隆抗体。初步鉴定证明：该抗体具有稳定的种属特异性，可鉴别人与其它动物，特别是猴的红细胞，不与己知的血型特异性成分交叉，可凝集成人及脐带血的红细胞。
Using human erythrocyte membrane as antigen, BALB / C mice were immunized with a selective immunosuppressive procedure. The immunized mouse spleen cells were fused with SP2 / 0 myeloma cells, and 3 hybridomas secreting anti-human erythrocyte membrane-specific monoclonal antibodies (M1A7C4) were screened by hemagglutination. M3A5B7 and M3D9F1. The hybridoma that has been continuously passaged for 2 months and resuscitated for 6 months can still stably secrete anti-human erythrocyte membrane monoclonal antibody. Preliminary identification shows that the antibody has a stable species-specific, can be identified in humans and other animals, especially monkey red blood cells, do not cross the known blood-specific components can be agglutinated adult and cord blood red blood cells.