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目的:探讨子宫颈癌发生中影响细胞凋亡的调节因素。方法:检测对象为194例经福尔马林固定石蜡包埋的手术切除标本,包括上皮内瘤样病变(CIN)78例犤其中重度非典型增生(SD)41例;原位癌(CIS)37例犦早期浸润癌(MIC)35例;大细胞非角化型浸润癌(IC)40例及正常宫颈鳞状上皮(NE)41例。凋亡细胞的检出使用TDT-mediateddUTP-biotinnickendlabeling(TUNEL)方法;细胞凋亡相关基因蛋白p53、bcl-2、bax的表达采用单克隆抗体免疫组化ABC染色方法;HPV16、18型E6DNA感染使用PCR方法进行检测。结果:1)凋亡细胞标记率仅在CIN呈现有意义地进行性减少(P<0.01),癌变以后不再继续下降;2)在SD、CIS组,p53、bcl-2及bax基因蛋白的表达与TUNEL标记率呈现有意义地相关性变化(P<0.05);3)HPV16、18型E6DNA感染与凋亡细胞及相关基因蛋白表达未呈现相关性改变。结论:以上结果提示细胞凋亡的改变与宫颈癌发生的早期过程有关,p53、bcl-2、bax基因蛋白参与其调节,而HPV16、18型E6DNA感染与其未呈现直接的相关性。
Objective: To investigate the factors affecting the apoptosis of cervical cancer. Method: detection target 194 cases of formalin-fixed paraffin-embedded surgical specimens, including intraepithelial neoplasia (CIN) 78 Pai embodiment wherein severe dysplasia (SD) 41 cases; carcinoma in situ (CIS) 37 cases of early invasive carcinoma (MIC) 35 cases; 40 cases of large cell non-keratinizing invasive carcinoma (IC) and 41 cases of normal cervical squamous epithelium (NE). Apoptosis detection using TDT-mediateddUTP-biotinnickendlabeling (TUNEL) method; apoptosis-associated gene protein p53, bcl-2, bax expression using monoclonal antibody immunohistochemistry ABC staining method; infection using E6DNA type of HPV16,18 PCR method to detect. Results: 1) Apoptotic cells markedly decreased only in CIN (P <0.01), and no further decline after canceration; 2) In the groups of SD, CIS, p53, bcl-2 and bax (P <0.05). (3) The expression of HPV16,18 and E6 DNA did not correlate with the expression of apoptotic cells and related genes. Conclusion: These results suggest that early in the process of apoptosis related changes of cervical cancer, p53, bcl-2, bax gene protein involved in their regulation, and the infection therewith HPV16,18 type E6DNA not exhibit a direct correlation.