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以１－（２－噻唑偶氮）－２－萘酚（ＴＡＮ）作柱前显色剂，于ＯＤＳ柱上，用内含０．１ｍｏｌ／ＬＬｉＣｌ，５×１０－６ｍｏｌ／Ｌ ＴＡＮ和ＨＡｃ－ＮＨ４Ａｃ缓冲溶液（ｐＨ ５．５）的甲醇－水溶液（８０：２０，Ｖ／Ｖ）作流动相，流速为０．６ｍＬ／ｍｉｎ，并以紫外－可见检测器于５９０ｎｍ处进行检测，发展了一种ＲＰ－ＨＰＬＣ法同时分离测定铜（Ⅱ）、铁（Ⅱ）、镍（Ⅱ）的方法，方法灵敏度高，对于铜、铁、镍的检测限分别为１μｇ／Ｌ， ２ μｇ／Ｌ和 ０．４ μｇ／Ｌ。用于实际样品测定，结果满意。
The column was developed on the ODS column with TAN (1-thiazolylazo) -2-naphthol (TAN) NH4Ac buffer solution (pH 5.5) in methanol-water solution (80:20, V / V) was used as the mobile phase at a flow rate of 0.6 mL / min and UV detection was performed at 590 nm. The method for the simultaneous determination of copper (Ⅱ), iron (Ⅱ) and nickel (Ⅱ) by RP-HPLC has high sensitivity and the detection limits are 1μg / L, 2μg / L and 0 .4 μg / L. For the actual sample determination, the results are satisfactory.