目的:观察钩藤碱固体脂质纳米粒(rhynchophylline solid lipid nanoparticles, Rhy-SLN)对小鼠气道平滑肌细胞增殖及TGF-β1/ERK/P38信号通路的影响。方法:小鼠气道平滑肌细胞体外分离培养，按随机数字表法分为空白组、TGF-β1组、Rhy-SLN组、抑制剂组。除空白组外，其余各组气道平滑肌细胞以0.2% BSA/DMEM无血清培养基培养，使细胞处于增殖停滞状态。TGF-β1组加入5 μg/L的TGF-β1进行干预；Rhy-SLN组加入5 μg/L的TGF-β1及10 μmol/L的Rhy-SLN进行干预；抑制剂组加入5 μg/L的TGF-β1及10 μmol/L SB431542进行干预。干预后24 h，采用MTT法检测小鼠气道平滑肌细胞增殖情况；采用Western blot法检测小鼠气道平滑肌细胞的增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)、ERK1/2、p-ERK1/2、p38、p-p38蛋白表达水平。结果:与TGF-β1组比较，Rhy-SLN组和抑制剂组吸光度值[(1.352±0.14)、(1.139±0.12)比(1.780±0.18)]降低(n P<0.05)，PCNA蛋白[(0.25±0.03)、(0.24±0.02)比(0.49±0.05)]、p-ERK1/2[(1.01±0.11)、(0.97±0.09)比(1.86±0.17)]、p-p38蛋白[(0.26±0.03)、(0.22±0.02)比(0.41±0.04)]表达降低(n P<0.05)。n 结论:Rhy-SLN可抑制小鼠气道平滑肌细胞增殖，其机制可能与调节TGF-β1/ERK1/2信号通路有关。“,”Objective:To observe the effect of rhynchophylline solid lipid nanoparticles (rhy SLN) on the proliferation of airway smooth muscle cells (ASMCs) and TGF-β1/ERK/p38 signaling pathway.Methods:The ASMCs of mice were isolated and cultured in vitro and randomly divided into blank group, TGF-β1 group, rhy SLN group and inhibitor group. In addition to the blank group, the other groups of airway smooth muscle cells were cultured in 0.2% BSA/DMEM serum-free medium, resulting in cell proliferation arrest. The blank group was healthy ASMCs; the TGF-β1 group was treated with 5 μg/L TGF-β1 for 24 hours; the rhy SLN group was treated with 5 μg/L TGF-β1 and 10 μmol/L rhy SLN for 24 hours; the inhibitor group was treated with 5 μg/L TGF-β1 and 10 μmol/L SB431542 for 24 hours. The proliferation of ASMCs was detected by MTT method, and the expression of proliferating cell nuclear antigen (PCNA) and TGF-β1/ERK/p38 pathway related proteins was detected by Western blot.Results:Compared with TGF-β1 group, the absorbance value (1.352 ± 0.14, 1.139 ± 0.12n vs. 1.780 ± 0.18) of the rhy SLN group and inhibitor group significantly decreased (n P<0.05), the expression of PCNA (0.25 ± 0.03, 0.24 ± 0.02n vs. 0.49 ± 0.05), p-ERK1/2 (1.01 ± 0.11, 0.97 ± 0.09 n vs. 1.86 ± 0.17), p-p38 (0.26 ± 0.03, 0.22 ± 0.02 n vs. 0.41 ±0.04) significantly decreased (n P<0.05).n Conclusion:Rhy SLN could inhibit the proliferation of ASMCs of mice, and its mechanism may be related to the regulation of TGF-β1/ERK1/2 signal pathway.