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作者将重度感染疟原虫子孢子的斯氏按蚊(印度株)26只,分为2组,每组各分别饱吸已切除脾脏的夜猴Ao-073和Ao-176的血液。在实验开始前的30天内,对各猴作数次血涂片检查疟原虫。在接种的前一天,给Ao-073腹腔注射1毫升免疫抑制剂——硫唑嘌吟(Imuran,每毫升含50毫克)和以后6周给予口服0.2克dl-蛋氨酸(Pedameth),每周5天。Ao-176仅用硫唑嘌吟。2天后,对1毫升冷却蚊虫培养基内尚存活的蚊虫进行涎腺解剖,将0.5毫升捣碎的含有子孢子的涎腺给各猴静脉接种,而后隔日作血涂片检查疟原虫。
Sixty-six Anopheles spp. (Indian strain), which were highly infected with sporozoites of Plasmodium, were divided into two groups, each of which was filled with the blood of Ao-073 and Ao-176, which had been spleen-excised. Within 30 days prior to the start of the experiment, blood tests were performed on each monkey to examine the parasite. On the day before inoculation, 1 ml of immunosuppressant Imuran (50 mg per ml) was intraperitoneally injected to Ao-073 and 0.2 g of dal-methionine (Pedameth) was administered orally 6 weeks later day. Ao-176 only with azathioprine Yin. Two days later, salivary gland dissection was performed on 1 ml of live mosquitoes in a cooling mosquito culture medium, 0.5 ml of mashed sporozoites containing sporozoites were inoculated into each vein, and blood smear was performed every other day to examine the parasite.