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本文描述了在人成纤维(HFS)细胞中增殖的甲型肝炎疫苗候选株的生物物理、生化和电镜观察的研究结果。用放射免疫法(RIA)检测HAV抗原,用微量板孔培养的HFS细胞滴定HAV感染性(TCID_(50)),用12烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定HAV蛋白质的分子量。含HAV的上清液取自HAV感染的HFS细胞培养液,待RIA检测HAV抗原滴度达1∶7时,每周收获一次。经硫酸铵沉淀、超速离心6500倍浓缩后,HAV的RIA抗原滴度达1∶
This article describes the results of biophysical, biochemical, and electron microscopic observations of Hepatitis A vaccine candidates that proliferate in human fibroblasts (HFS) cells. HAV antigen was detected by radioimmunoassay (RIA), HAV infectivity (TCID_ (50)) was measured on HFS cells cultured in microplate wells, and HAVs were determined by sodium 12 alkyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) The molecular weight of a protein. The HAV-containing supernatant was taken from HAV-infected HFS cell culture and harvested weekly until RIA detected a 1: 7 HAV antigen titer. After ammonium sulfate precipitation and ultracentrifugation 6500-fold enrichment, the HAV RIA antigen titers reached 1: