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目的 观察D 半乳糖连续皮下注射对小鼠皮肤衰老指标的影响。 方法 3月龄KM雌性小鼠 6 0只 ,随机分为空白对照组 (注射生理盐水 )、D 半乳糖低剂量组 (80mg/kg)和D 半乳糖高剂量组 (10 0 0mg/kg) ,连续每日背部皮下注射 4 2天后 ,观察小鼠皮肤中与衰老相关的生化指标及组织病理学变化 ,并用计算机图像分析系统定量分析。 结果 高剂量组小鼠真皮厚度〔(6 2 4 5±4 8 5 ) μm〕较对照组〔(839 3± 5 8 4 ) μm〕显著变薄 (P <0 0 5 ) ,胶原纤维减少 ,排列疏松 ,弹力纤维总面积亦显著减少 (P <0 0 5 ) ;高剂量组皮肤中超氧化物歧化酶 (SOD)活性〔(131 2± 11 5 )U/ml〕、羟脯氨酸含量〔(0 5 7± 0 13) μg/mg〕显著低于对照组的〔(178 1± 2 0 7)U/ml、(0 74± 0 17) μg /mg ,P <0 0 5〕 ,丙二醛 (MDA)含量增加〔(9 4± 1 5 )nmol/ g和 (6 8± 1 5 )nmol/ g ,P <0 0 5〕 ;而低剂量组上述改变则不显著。 结论 每日 10 0 0mg/kgD 半乳糖连续皮下注射 4 2天 ,可导致小鼠皮肤的明显衰老 ,为抗皮肤衰老研究提供一个简便、稳定的实验模型。
Objective To observe the effect of D-galactose continuous subcutaneous injection on mouse skin aging index. Methods Sixty female KM mice of 3 months old were randomly divided into blank control group (injected with normal saline), D-galactose low dose group (80mg / kg) and D galactose high dose group (100 mg / kg) After continuous subcutaneous injection of 4 days daily, the biochemical indexes and histopathological changes related to aging in mouse skin were observed and quantified by computer image analysis system. Results Compared with the control group [(839 3 ± 5 8 4) μm], the thickness of the dermis in the high-dose group [(6 2 4 5 ± 4 8 5) μm] was significantly thinner (P 0 05) (P <0 05); the activity of superoxide dismutase (SOD) in the skin of high-dose group [(131 2 ± 11 5) U / ml), the content of hydroxyproline (0 57 ± 0 13) μg / mg) were significantly lower than those in the control group (178 1 ± 2 0 7 U / ml, 0 74 ± 0 17 μg / mg, P 0 05) The levels of MDA increased (9 4 ± 1 5) nmol / g and (6 8 ± 1 5) nmol / g, respectively, P 0 05), while those in the low dose group did not change significantly. Conclusion Continuous subcutaneous injection of galactose at a dose of 10 0 mg / kgD for 42 days can result in significant skin aging in mice, providing a simple and stable experimental model for skin anti-aging research.