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目的 探讨大鼠蛛网膜下腔出血 (SAH)后局部脑血流 (rCBF)与血脑屏障 (BBB)超微结构的动态变化规律和尼莫地平 (Nim)的作用特点。方法 通过大鼠枕大池自体血注入法制备SAH后脑血管痉挛 (CVS)模型 ,在SAH后 1h、 4h、 12h、 2 4h、 3d、 7d、 14d及 2 1d等不同时相点 ,利用激光多普勒技术和电镜对各组大鼠局部脑血流与血脑屏障超微结构变化进行观察。结果 SAH后大鼠的rCBF明显下降且具有“两期”反应 ,在SAH后 1h后rCBF有所恢复 ,4h后再度降低 ,并可持续至 2 1d。BBB损害改变在SAH后早期即可观察到。Nim对rCBF降低和BBB损害均有改善作用。结论 SAH后rCBF与BBB超微结构的动态变化符合CVS的发展规律 ,可以反映该时期CVS的状态。早期应用Nim可以改善SAH后CVS ,并对BBB的损害有一定保护作用
Objective To investigate the dynamic changes of ultrastructure of regional cerebral blood flow (rCBF) and blood-brain barrier (BBB) in rats after subarachnoid hemorrhage (SAH) and the effect of nimodipine (Nim). Methods Cerebral vasospasm (CVS) model of SAH was established by autologous blood perfusion into the cistern and cisternus of rats. At different time points of 1h, 4h, 12h, 24h, 3d, 7d, 14d and 21d after SAH, Le technical and electron microscopy of the regional cerebral blood flow in rats and changes in the blood-brain barrier ultrastructure were observed. Results The rCBF of rats decreased significantly after SAH and had a “two-phase” response. After 1 h SAH, rCBF recovered, and then decreased again after 4 h and lasted for 21 days. Changes in BBB damage can be observed early after SAH. Nim improves rCBF reduction and BBB damage. Conclusion The dynamic changes of ultrastructure of rCBF and BBB after SAH accord with the development of CVS, which can reflect the state of CVS during this period. Early application of Nim can improve CVS after SAH, and have a protective effect on BBB damage