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目的建立不同产地中药材水线草与白花蛇舌草的HPLC特征图谱,用以区分水线草与白花蛇舌草。方法采用HPLC分析,色谱柱为Agilent Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm),以甲醇-水为流动相系统进行梯度洗脱,流速1.0 m L·min~(-1),检测波长254 nm,柱温30℃;采用《中药指纹图谱相似度评价系统》进行相似度评价;采用Matlab7.0软件进行主成分分析和聚类分析。结果分别得到水线草和白花蛇舌草的HPLC特征图谱,经比较,两者共有峰为8个,分别为a~h;特征峰水线草中峰为7~12,白花蛇舌草中峰为1~6;相似度分析结果显示,不同来源水线草相似度值在0.733~0.984之间,不同来源白花蛇舌草相似度值在0.873~0.951之间;主成分分析和聚类分析将供试药材分为3类:自采水线草、自采白花蛇舌草及购买白花蛇舌草。结论该方法可以快速简便地区分中药材水线草和白花蛇舌草,可为两者的质量控制及临床使用提供参考。
OBJECTIVE To establish the HPLC characteristic map of Chinese herbal medicine Herbarium and Hedyotis diffusa in different areas to distinguish between Herba Isatis and Hedyotis diffusa. Methods The HPLC elution was carried out on a Agilent Eclipse XDB-C 18 column (4.6 mm × 250 mm, 5 μm) using a mobile phase of methanol-water as the mobile phase at a flow rate of 1.0 m L · min -1 , The detection wavelength was 254 nm and the column temperature was 30 ℃. The similarity was evaluated by using the similarity evaluation system of fingerprint of Chinese traditional medicine. The Matlab7.0 software was used to carry out principal component analysis and cluster analysis. Results The HPLC profiles of waterfly and Hedyotis diffusa were obtained, respectively. The results showed that there were 8 peaks in total, which were a ~ h, respectively. The peak of characteristic peakline was 7-12, The results of similarity analysis showed that the similarity values of watercress from different origins ranged from 0.733 to 0.984 and the similarity values from different origins of Hedyotis diffusa were from 0.873 to 0.951. The principal component analysis and cluster analysis The test herbs are divided into three categories: since the water line grass, since the Hedyotis diffusa and the purchase of Hedyotis diffusa. Conclusion This method can quickly and easily distinguish Chinese herbal medicine and Hedyotis diffusa, which can provide reference for both quality control and clinical use.