论文部分内容阅读
目的:观察不同程度妊娠期高血压疾病母儿脐血神经元特异性烯醇化酶(NSE)和S-100蛋白水平及其变化、NSE和S-100蛋白间的关系及二者与1 min Apgar评分的相关性,探讨母亲妊娠期高血压疾病对胎儿脑细胞代谢的影响,为早期诊断、及时处理新生儿脑损伤提供可靠的客观依据。方法:采用放射免疫法检测妊娠期高血压疾病母亲所生的69例新生儿及20例对照组新生儿脐血NSE和S-100蛋白水平并进行分析比较。结果:妊娠期高血压疾病组、轻度子痫前期组、重度子痫前期组、正常对照组脐血NSE/S-100蛋白含量分别为(9.77±1.34)ng/mL/(37.50±2.52)μg/L、(9.70±1.37)ng/mL/(38.84±5.43)μg/L、(11.33±1.70)ng/mL/(60.50±10.04)μg/L、(8.82±1.10)ng/mL/(35.32±3.38)μg/L,4组之间比较差异有统计学意义(P<0.05);妊娠期高血压疾病母儿脐血NSE与S-100蛋白水平呈正相关(γ=0.457,P<0.05);妊娠期高血压疾病母儿脐血NSE、S-100蛋白水平与新生儿1 min Apgar评分均呈负相关(γ=-0.286/γ=-0.263,P<0.05)。结论:妊娠期高血压疾病程度越重,脐血NSE与S-100蛋白水平升高越明显,造成围产儿脑损伤的可能性越大。妊娠期高血压疾病母儿脐血NSE、S-100蛋白水平与1 min Apgar评分呈负相关,说明脐血NSE、S-100蛋白可以做为预测新生儿脑损伤的客观指标。
Objective: To observe the levels of neuron specific enolase (NSE) and S-100 protein in maternal and umbilical cord blood, and the relationship between NSE and S-100 protein in hypertensive disorders of pregnancy and their relationship with 1 min Apgar Score, explore the impact of maternal hypertensive disorders on fetal brain cell metabolism in order to provide a reliable and objective basis for early diagnosis and timely treatment of neonatal brain injury. Methods: Radioimmunoassay was used to detect the levels of NSE and S-100 in cord blood of 69 neonates with gestational hypertension and 20 controls. Results: The levels of NSE / S-100 protein in umbilical blood of gestational hypertension group, mild preeclampsia group, severe preeclampsia group and normal control group were (9.77 ± 1.34) ng / mL and (37.50 ± 2.52) (9.70 ± 1.37) ng / mL / (38.84 ± 5.43) μg / L, (11.33 ± 1.70) ng / mL / (60.50 ± 10.04) μg / L and (8.82 ± 1.10) ng / mL / 35.32 ± 3.38) μg / L, there was a significant difference between the 4 groups (P <0.05). There was a positive correlation between NSE and S-100 protein levels in maternal hypertensive disorders (γ = 0.457, P <0.05 ). The levels of NSE and S-100 protein in maternal and umbilical cord blood of patients with gestational hypertension were negatively correlated with Apgar score at 1 minute (γ = -0.286 / γ = -0.263, P <0.05). Conclusion: The more severe the hypertensive disorders in pregnancy, the more obvious the increase of NSE and S-100 protein levels in umbilical cord blood, the greater the possibility of perinatal brain injury. The levels of NSE and S-100 in maternal and umbilical cord blood of patients with gestational hypertension were negatively correlated with 1 min Apgar scores, indicating that umbilical cord blood NSE and S-100 protein could be used as objective indicators to predict neonatal brain injury.