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目的 探讨 1997年夏深圳地区暴发流行的急性咽结膜炎的病毒病原学。方法 采用急性咽结膜炎患儿急性期眼、咽拭子进行病毒分离 ,并同时用PCR法检查标本中病毒及分离病毒鉴定 ,对分离出部分毒株采用多个内切酶进行内切酶分析。此外 ,还测定患儿双份血中病毒的中和抗体增长。结果 14份患儿咽拭子和 14份眼拭子病毒分离阳性率分别为 71 43%和 6 4 2 9% ;PCR法检测标本中病毒DNA的检出率分别为 78 5 7%和 71 43%。所有分离病毒株均为 3型腺病毒。选择其中 3对 (咽和眼拭子分离的毒株 )病毒株。内切酶分析显示均为Ad3a2基因型。 2 4例患儿双份血清均显示抗体效价≥ 4倍增长。分离株与其自身急性期和恢复期血清的中和试验也显示出恢复期中和抗体均较急性期有≥ 4倍增长。结论 本次急性咽结膜炎暴发流行是由 3型腺病毒引起。
Objective To explore the viral etiology of acute pharyngoconjunctivitis outbreak in Shenzhen in the summer of 1997. Methods Acute pharyngoconjunctivitis children with acute phase of eye and throat swab virus isolation, and at the same time by PCR method to check the samples of virus and isolated virus identification, the isolation of some strains using multiple endonuclease enzyme analysis . In addition, the determination of the virus in children with double copies of the neutralizing antibody growth. Results The positive rates of pharyngeal swabs and 14 eye swabs isolated from 14 children were 71 43% and 64 2% respectively. The detection rates of viral DNA in PCR specimens were 78 57% and 71 43 %. All isolated virus strains are type 3 adenoviruses. Three pairs of strains (strains isolated from pharyngeal and eye swabs) were selected. Endonuclease analysis showed the Ad3a2 genotype. Twenty-four children with double serum showed antibody titers> 4-fold increase. Neutralization of the isolates with their own acute and convalescent sera also showed a> 4-fold increase in neutralizing antibodies during the recovery phase compared to the acute phase. Conclusion The outbreak of acute pharyngoconjunctivitis epidemic is caused by adenovirus type 3.