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利用滴涂于铂盘表面的 Nation 膜中负电性的磺酸基与 anti-IgG 分子中的氨基阳离子之间的静电作用实现抗体的结合,同时通过负电性的纳米金增加抗体的固定量,最后在修饰电极的表面涂敷一层明胶(Gelatin)薄膜进行固定,制成 Gelatin/anti-IgG/Au/Nafion/Pt 膜,制得非标记免疫传感器.用循环伏安法(CV)和交流阻抗法(Nyquist)对电极逐层修饰过程进行了表征,并对免疫传感器的性能进行了研究.该传感器测定 IgG 的最低浓度为2.0μg/L,标准曲线的线性范围在5～960μg/L,回归方程为:ΔE=-2.2+31.7 log[IgG],响应时间为5 min.此传感器制备过程简单、稳定性好、灵敏度高、具有良好的选择性,用于生物样品分析,结果令人满意。
Antibody binding is achieved by the electrostatic interaction between the negatively charged sulfonic acid group in the Nation membrane dripped on the surface of the platinum plate and the amino cation in the anti-IgG molecule. At the same time, the amount of the immobilized antibody is increased by the negatively charged nano-gold. Gelatin / anti-IgG / Au / Nafion / Pt films were coated with a layer of gelatin film on the surface of the modified electrode to prepare a non-labeled immunosensor. Cyclic voltammetry (CV) and alternating current impedance Nyquist was used to characterize the electrode layer-by-layer modification process and the performance of the immunosensor was studied.The minimum concentration of IgG detected by this sensor was 2.0 μg / L, the linear range of the standard curve was 5 ~ 960 μg / L, The equation is: ΔE = -2.2 + 31.7 log [IgG], the response time is 5 min.This sensor has a simple preparation process, good stability, high sensitivity and good selectivity for biological sample analysis with satisfactory results.