Gene cloning of human soluble CD14 and its expression ineucaryotic cells

来源 :中华创伤杂志：英文版 | 被引量 : 0次 | 上传用户：sky_bj

【摘要】

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To express human soluble CD14 (sCD14) in eukaryotic cells.Methods: Human sCD14 cDNA was amplified from U937 cells with RT-PCR method. The recombinant expression

【作者】

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荫俊白洁王威宋伟王忠泽

【机构】

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BeijingInstituteofMicrobiology

【出处】

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中华创伤杂志：英文版

【发表日期】

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2002年3期

【关键词】

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基因克隆可溶性CD14 基因表达序列分析 COS-7细胞 CD Gene Eucaryotic expression Biological funct

【基金项目】

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国家自然科学基金

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To express human soluble CD14 (sCD14) in eukaryotic cells.Methods: Human sCD14 cDNA was amplified from U937 cells with RT-PCR method. The recombinant expression plasmid pEF1/HisC/sCD14348aa was constructed and the expression in COS-7 cells was carried out

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Gene cloning of human soluble CD14 and its expression ineucaryotic cells

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