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目的研究银杏叶提取物(EGb)对大鼠急性脊髓损伤后运动功能的恢复及脊髓Nogo-A蛋白表达的影响。方法选用健康的成年Sprague-Dawley(SD)大鼠72只,雌雄不拘,体质量250~300 g。将大鼠分为正常组、急性脊髓损伤组和急性脊髓损伤后EGb治疗组(简称EGb治疗组)。采用Allen’s打击方法损伤大鼠胸椎T_8~T_(10)节段,分别于术后3、7、21 d进行BBB评分和斜板实验,并取受损节段大鼠脊髓,运用Western blot方法测定各时相点Nogo-A蛋白表达量及其变化。结果术后21 d EGb治疗组比急性脊髓损伤组BBB评分和斜板实验临界角度升高,差异有统计学意义(P<0.05)。Nogo-A蛋白在各组大鼠脊髓组织中均呈阳性表达,急性脊髓损伤组与EGb治疗组各个时相点Nogo-A蛋白表达量均显著高于正常组(P<0.05),7 d时最高,21 d有所下降,但表达量仍高于正常组。EGb治疗组在各个时间点的NogoA表达量显著低于急性脊髓损伤组,差异有统计学意义(P<0.05)。结论 EGb可促进急性脊髓损伤后大鼠运动功能的恢复,对脊髓损伤后Nogo-A蛋白表达量的升高具有明显的抑制作用。
Objective To investigate the effects of Ginkgo biloba extract (EGb) on the recovery of motor function and Nogo-A protein expression in spinal cord after acute spinal cord injury in rats. Methods Seventy two healthy adult Sprague-Dawley (SD) rats were chosen, both male and female, weighing 250-300 g. Rats were divided into normal group, acute spinal cord injury group and EGb treatment group (abbreviated EGb treatment group) after acute spinal cord injury. Using Allen’s method, rat’s thoracic T_8 ~ T_ (10) segments were injured, BBB score and oblique plate test were performed at 3, 7, and 21 days after operation, respectively. The expression of Nogo-A protein and its changes at different time points. Results The BBB score and the critical angle of inclined plate experiment in EGb-treated group were significantly higher than those in acute spinal cord injury group 21 d after operation (P <0.05). The expression of Nogo-A protein in spinal cord tissue of each group was significantly higher than that of the normal group (P <0.05). The expression of Nogo-A protein at each time point was significantly higher in the acute spinal cord injury group and the EGb-treated group than in the normal group The highest, 21 d decreased, but the expression level is still higher than the normal group. The expression of NogoA in EGb group was significantly lower than that in acute spinal cord injury group at each time point, the difference was statistically significant (P <0.05). Conclusion EGb can promote the recovery of motor function of rats after acute spinal cord injury and obviously inhibit the increase of Nogo-A protein after spinal cord injury.