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[目的]采用反相高效液相色谱内标法测定25%丙溴磷·灭多威乳油中丙溴磷、灭多威的含量。[方法]使用Diamonsil C_8色谱柱,以甲醇-水(体积比为70∶30)为流动相,232 nm为紫外检测器检测波长,对羟基苯甲酸丙酯为内标物进行测定。[结果]丙溴磷、对羟基苯甲酸丙酯和灭多威的保留时间分别为10.0、6.5、3.2 min,丙溴磷和灭多威的线性相关系数分别为0.9997和0.9990,变异系数分别为0.63%和1.19%,平均回收率分别为98.87%和98.23%。[结论]方法简便、结果准确、重现性好。
[Objective] To determine the contents of profenofos-methyl and methomyl in 25% profenofos-methyl and methylglucoside by RP-HPLC. [Method] With Diamonsil C_8 column, the mobile phase was methanol - water (volume ratio is 70:30), the detection wavelength was at 232 nm and the propyl p - hydroxybenzoate was used as internal standard. [Results] The retention times of profenofos, propylparaben and methomyl were 10.0, 6.5 and 3.2 min, respectively. The linear correlation coefficients of profenofos and methomyl were 0.9997 and 0.9990 respectively, and the coefficients of variation were 0.63% and 1.19% respectively. The average recoveries were 98.87% and 98.23% respectively. [Conclusion] The method is simple, the result is accurate and the reproducibility is good.