论文部分内容阅读
目的研究胰头癌患者外周血中CD4+CD25+调节性T细胞(Treg)比例及其在手术前、后的变化趋势。方法采用流式细胞仪检测胰头癌患者及正常对照组外周血中CD4+CD25+调节性T细胞的数量,同时监测CD4+/CD8+比值,并进行手术前、后的比较。结果胰头癌患者术前外周血中CD4+CD25+和CD4+CD25highTreg所占比例较正常对照组高(P<0.05),术后则出现不同程度下降,以术后第3天下降最明显(P<0.01,P<0.05);胰头癌患者术后CA19-9水平低于术前,以术后第14天下降明显(P<0.05)。CD4+CD25highTreg与CA19-9的变化趋势大致相同。胰头癌患者术前CD4+/CD8+比值比正常对照组低(P<0.05),手术后进一步降低,于手术后第7天达最低(P<0.05)。结论胰十二指肠切除术可能有助于机体抗肿瘤免疫的恢复,胰头癌患者围手术期可作为免疫干预的重要窗口期,CD4+CD25+调节性T细胞可作为免疫干预的靶点。
Objective To study the proportion of CD4 + CD25 + regulatory T cells (Tregs) in peripheral blood of patients with pancreatic head carcinoma and their changes before and after operation. Methods The number of CD4 + CD25 + regulatory T cells in peripheral blood of patients with pancreatic head cancer and normal control group was detected by flow cytometry. The ratio of CD4 + / CD8 + was also monitored and compared before and after surgery. Results The proportion of CD4 + CD25 + and CD4 + CD25high Treg in peripheral blood of patients with pancreatic head cancer before operation was significantly higher than that of the control group (P <0.05) and decreased to some extent postoperatively (P <0.01, P <0.05). The level of CA19-9 in patients with pancreatic head cancer was significantly lower than that before operation and decreased significantly on the 14th day after operation (P <0.05). The trends of CD4 + CD25highTreg and CA19-9 are roughly the same. The preoperative CD4 + / CD8 + ratio of patients with pancreatic head cancer was significantly lower than that of the normal control group (P <0.05), and decreased further after operation, reaching the lowest on the 7th day after operation (P <0.05). Conclusions Pancreaticoduodenectomy may contribute to the recovery of anti-tumor immunity. Pancreatic cancer patients may be used as an important window for immune intervention. CD4 + CD25 + regulatory T cells may serve as targets for immune intervention.