目的比较致死样哮喘(NFA)缓解期患者与健康人中常见的呼吸道病毒的分布差异。方法研究对象包括NFA缓解期患者12例,轻症哮喘患者21例及无哮喘病史的健康人52名。诱导痰用于RNA及DNA的提取,PCR及RT-PCR方法用于7种常见的呼吸道病毒,微小病毒(HRV),流感病毒A、B,副流感病毒1、3,呼吸道合胞病毒及腺病毒的检测。结果NFA缓解期患者的病毒检出率为42%(5/12),而轻症患者为24%(5/21),健康人为10%(5/52)。NFA患者的病毒检出率显著高于健康人。微小病毒、流感病毒A、B可存在于NFA缓解期患者的下呼吸道内。而健康人呼吸道内以微小病毒为主。哮喘患者气道内存在2种以上病毒定植的情况。结论呼吸道病毒可定植于哮喘患者及健康人的气道内而不诱发呼吸道症状,呼吸道病毒定植可能与哮喘的严重度有关。 Objective To compare the distribution of common respiratory viruses in patients with nausea-like asthma (NFA) remission and healthy individuals. Methods The subjects included 12 patients with NFA remission, 21 with mild asthma and 52 healthy individuals without a history of asthma. Induction of sputum for RNA and DNA extraction, PCR and RT-PCR methods for seven common respiratory viruses, parvovirus (HRV), influenza viruses A, B, parainfluenza virus 1, respiratory syncytial virus and adenovirus Virus detection. Results The detection rate of virus in NFA patients was 42% (5/12), compared with 24% (5/21) in mild patients and 10% (5/52) in healthy subjects. The detection rate of virus in NFA patients is significantly higher than that in healthy people. Parvovirus, influenza A, B may be present in the lower respiratory tract of patients with NFA remission. The healthy people in the respiratory tract with the main parvovirus. There are two or more types of virus colonization in the airway of asthmatic patients. Conclusion Respiratory viruses can be colonized in the airways of asthmatic patients and healthy people without inducing respiratory symptoms. Respiratory virus colonization may be related to the severity of asthma.