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目的:探索当归对四氯化碳(CCl4)肝损伤的保护作用。方法:50只小鼠随机分成5组:对照组(Ⅰ)、模型组(Ⅱ)、当归低剂量组(Ⅲ)、中剂量组(Ⅳ)和高剂量组(Ⅴ)。Ⅲ~Ⅴ组分别按5,10,20 g.kg-1剂量sc当归水提醇沉液,Ⅰ、Ⅱ组注射等容量的生理盐水,每天1次,连续6 d。末次给药后2 h,Ⅱ~Ⅴ组ip 0.1%CCl4四氯化碳石蜡油溶液(0.1 mL.10g-1体重),24 h后处死小鼠,电镜观察肝组织结构线粒体和内质网变化。结果:当归各组肝细胞内线粒体及内质网结构受损伤程度较模型组明显减轻。结论:当归水提醇沉液对CCl4致小鼠急性肝损伤具有一定的保护作用。
Objective: To explore the protective effect of Angelica sinensis on carbon tetrachloride (CCl4) liver injury. Methods: Fifty mice were randomly divided into five groups: control group (Ⅰ), model group (Ⅱ), low dose group of Angelicae sinensis (Ⅲ), middle dose group (Ⅳ) and high dose group (Ⅴ). Groups Ⅲ and Ⅴ were treated with 5, 10 and 20 g · kg-1 doses of Angelica Sinensis to extract alcohol-precipitated fluid respectively. Rats in groups Ⅰ and Ⅱ were injected with the same volume of normal saline once a day for 6 days. At 2 h after the last administration, mice in groups Ⅱ and Ⅴ were treated with ip 0.1% CCl 4 CCl 4 paraffin oil (0.1 mL, 10 g-1 body weight) for 24 h. The changes of mitochondria and endoplasmic reticulum . Results: The damage of mitochondria and endoplasmic reticulum in hepatocytes of Angelica sinensis group was significantly reduced compared with model group. Conclusion: Angelica water extract alcohol precipitation of CCl4 induced acute liver injury in mice has a protective effect.