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目的:探讨小鼠载脂蛋白E(apoE)基因的组织表达特点。方法:提取小鼠的肝、脑和心等17个组织的RNA,采用RTPCR的方法对apoE基因在上述组织中的表达进行研究。结果:apoE基因在肝组织表达最强,其次是脊髓和脑,肺、肾、心、脾、胸腺、小肠、胃、膀胱、血液、肾上腺、子宫、胰腺和主动脉也有不同程度的表达,但在腹肌中几乎不表达。结论:小鼠的apoE基因组织表达谱与人的apoE基因组织表达谱有较高的一致性,与猪的相比差异也不大。
Objective: To investigate the tissue expression characteristics of apoE gene in mice. Methods: The RNA of 17 tissues such as liver, brain and heart of mice was extracted and the expression of apoE gene in these tissues was studied by RTPCR. Results: The expression of apoE gene was the highest in liver tissue, followed by spinal cord and brain, lung, kidney, heart, spleen, thymus, small intestine, stomach, bladder, blood, adrenal gland, uterus, pancreas and aorta Almost not expressed in the abdominal muscles. CONCLUSION: The apoE gene expression profile of mice is highly consistent with the human apoE gene expression profile, but not significantly different from that of pigs.